Recombinant Protein Mammalian Expression Service
Gxene Biotech has conventional mammalian expression systems, including cell lines such as FreeStyle 293-F cell line (Thermo Fisher) and Expi CHO-S (Thermo Fisher). Combined with our designed expression vectors (containing a full-length CMV promoter and an optimized secretion signal peptide sequence), we can provide customers with recombinant protein mammalian cell expression and preparation at expression levels up to 1 g/L. Gxene Biotech has been dedicated to recombinant protein expression and preparation research for many years. Our scientists have rich experience in mammalian recombinant protein expression, fermentation, and purification, and can quickly develop customized mammalian cell protein expression strategies for different customers.
The mammalian protein expression system is suitable for scientists' in vitro recombinant expression needs for native-conformation proteins, such as glycosylation modification and phosphorylation modification. Using the mammalian protein expression system and high-density fermentation culture technology, Gxene Biotech's scientists can achieve recombinant antibody fermentation at expression levels up to 6.3 g/L, suitable for high-level expression fermentation of recombinant monoclonal antibody drugs. Our cell fermentation and culture personnel have all undergone strict GMP system training and quality control system training and can provide customers with traceable protein expression and preparation documentation records.
Gxene Biotech can provide customers with recombinant proteins produced in strict accordance with GMP, including cell seed bank source documentation records, cell seed lot testing, quality control of animal-derived components, standardized cell culture and transformation operating procedures, and a full set of GE-provided purification resins and equipment. For our large-scale fermentation, in addition to using fermenters, for 3–10 L adherent mammalian cell fermentation scales, we can directly use disposable cell culture factories for cell fermentation and protein expression preparation (customers need to provide lentivirus or adenovirus, etc.). This technology platform completes fermentation and transformation in one step, greatly shortening the turnaround time.
Service Flowchart
Service Content and Steps
|
Step |
Specific Service Content |
Turnaround |
|
Gene Cloning and Vector Construction |
*Amplify/identify gene fragments or synthesize the target gene, and insert it into a mammalian cell expression plasmid *Confirm the subcloned gene by PCR and sequencing Deliverables: sequencing data and experimental scheme |
1-2weeks |
|
Transient Protein Expression |
*Transfect cells with recombinant plasmid (293F, CHO): transient expression *SDS-PAGEand WB detection Deliverables: expression results and experimental report, 0.02–2 mg protein, purity >85% |
3–4 weeks |
|
Stable Expression (Optional) |
Construction and screening of recombinant protein CHO or 293F stable expression cell lines. Deliverables: stable cell line, construction report; 0.02–2 mg recombinant protein. |
4–6 weeks |
|
Small-Scale Protein Expression |
*Small-scale expression and expression condition optimization: 1 L small-scale protein expression. Deliverables: optimization report, culture conditions, and 1–5 mg recombinant protein. |
3–4 weeks |
|
Large-Scale Protein Preparation and Purification |
* 30L, 80 L, 130 L, and 250 L fermenters, as well as cell factories of various specifications (40 layers * N). *Purify the expressed recombinant protein by various chromatography methods such as affinity, ion exchange, hydrophobic interaction, and gel filtration. Deliverables: recombinant protein and purification report. The final protein can be provided with a purification tag (Tag) or with the purification tag (Tag) removed according to customer requirements. |
5–8 weeks |
|
Special Protein Expression |
*Customer provides designated cell line; Gxene Biotech performs recovery and scale-up culture. *Customized microcarrier suspension culture technology, suitable for high-density cell fermentation culture and protein expression of adherent cells (cell number: 10^7/ml). *Cell factory culture |
Negotiable |
Our Service Advantages
—Codon optimization: including rare codon analysis, protein hydrophilicity analysis, and predictive analysis of transmembrane domains
—Multiple fusion tag vectors and hosts to ensure high soluble protein expression and high protein activity
—High-activity expression: achieves complex glycosylation and phosphorylation modifications, with complete spatial structural characteristics
—Multiple expression modes: stable expression and transient expression
—Low endotoxin level: LAL assay <0.1 EU/µg
—Multiple scale protein fermentation modes: small scale (500 ml, 2.5 L, 10 L) and large scale (30 L, 80 L, 130 L, and 250 L fermenters)
—Complete GMP documentation support system, ensuring that all materials, reagents, and preparation information for your products are traceable
