Gxene Biotech has been engaged in molecular biology research for many years. Our molecular biology platform has a team of highly educated technical personnel with extensive experience in molecular biology operations and can provide you with professional molecular biology experimental services. High-quality plasmids are the foundation of molecular biology research. Endotoxins generated during plasmid extraction in cell transfection can also affect transfection efficiency and interfere with transfection results. Gxene Biotech can provide customers with routine plasmid extraction services and endotoxin-free plasmid preparation services according to their specific needs, to meet the needs of different subsequent experiments. At the same time, we can also provide one-stop services from plasmid extraction toprotein expression,antibody preparation, and other one-stop services.
Plasmids are small circularDNA molecules; they are DNA molecules that replicate autonomously independently of the bacterial chromosome. Most prokaryotic plasmids are double-stranded circularDNA molecules; however, linear plasmids can be found in both Gram-positive and Gram-negative bacteria. Plasmid size varies greatly, ranging from a few to hundreds ofkb. Plasmids rely on proteins provided by the host cell for replication, but they can also enable the host cell to acquire plasmid-encoded functions. Plasmid replication can be synchronized with the bacterial cell cycle, resulting in a low plasmid copy number in the bacterial cell; plasmid replication can also be independent of the cell cycle, allowing hundreds to thousands of plasmid copies to be amplified in each bacterial cell.
载体构建服务内容:
Service Details
Turnaround (Working Days)
Routine Plasmid Extraction
·Can meet routine PCR amplification,
sequencing, transformation, and other routine experiments
—Quality assurance: sequences are verified by restriction enzyme digestion and sequencing. All delivered plasmids have undergone 100% sequence verification