Protein Purification Service

Gxene Biotech has been striking on recombinant protein expression and antibody development for many years, and we understand the need for purification to produce clean, reproducible results. We can provide customers with customized protein purification services, including tag/tag free recombinant proteins, purification and separation of natural proteins. Our laboratory has more than a dozen GE purification resins that enable simultaneous large-scale scanning protein purification and separation, which can save your time and money. 

 
Gxene Biotech can provide natural and recombinant proteins purification services:
 
Natural Protein Purification Service:
 

Project Name	Protocol
Separation and purification of natural macromolecular proteins	Separation and purification proteins using molecular sieves of appropriate pore size, combined with anion and cation adsorption columns and HLPC, identify and collect corresponding elution peaks.
Separation and purification of natural small molecular proteins	Multi-layer separation and filtration combined with molecular sieve to remove macromolecular impurities, combined with macroporous resin or reverse resin to purify small molecular proteins, purified by HPLC and MS.
Separation and purification of mixed unknown protein samples	Separation and purification were carried out by KMD Bioscience, and identified by mass spectrometry, activity identification, etc.

 

Recombinant Protein Purification Services:
 

Project Name	Protocol
Deproteinization, separation and purification of recombinant proteins	Commercially purified resin, such as Ni column, GST-Beads, etc., is purified and purified with 6xHis, GST, SUMO, MBP to obtain a fusion protein with a purity > 90%.
Tag free recombinant protein purification	Antibody affinity purification; also can be selected similar to the natural protein purification method, depending on the project
Inclusion body denaturation and renaturation purification	According to the different properties of the protein, urea, guanidine hydrochloride, detergent, extreme pH, etc. can be selected to ensure the monomeric state's maximum denaturation.