活性重组蛋白 Recombinant Human TIM 3 / HAVCR2 / CD366 / KIM-3 / SPTCL Protein　重组蛋白

研究方向

Human TIM-3（T cell Immunoglobulin and Mucin domain-containing protein 3，T细胞免疫球蛋白和黏蛋白结构域蛋白3）是一种重要的免疫检查点分子，主要在T细胞、NK细胞和某些髓系细胞中表达。它在免疫调节中起关键作用，特别是在免疫耐受和免疫逃逸中。以下是关于Human TIM-3的详细介绍：

1. 基本结构与功能

结构：TIM-3是一种跨膜蛋白，其胞外部分包含免疫球蛋白样结构域和黏蛋白样结构域。
功能：TIM-3通过与其配体（如Galectin-9、CEACAM1等）结合，传递抑制性信号，调控免疫细胞的活化和功能。

2. 表达与分布

表达细胞：TIM-3主要在活化的T细胞、NK细胞、树突状细胞和巨噬细胞中表达。
组织分布：广泛分布于淋巴组织、外周血和肿瘤微环境中。

3. 免疫调节作用

免疫抑制：TIM-3通过抑制T细胞的活化和功能，参与免疫耐受和免疫逃逸。
抗炎作用：在炎症反应中，TIM-3帮助调控免疫反应的强度，防止过度的炎症损伤。
肿瘤免疫逃逸：在肿瘤微环境中，TIM-3的高表达与肿瘤细胞的免疫逃逸密切相关。

4. 临床意义

疾病关联：TIM-3的表达异常与多种疾病相关，包括癌症、自身免疫性疾病和感染性疾病。
治疗靶点：由于其在免疫调节中的重要作用，TIM-3被认为是免疫治疗的重要靶点，特别是在癌症和自身免疫性疾病的治疗中。

5. 研究进展

基础研究：目前的研究主要集中在TIM-3的配体识别机制、信号传导途径及其在疾病中的作用。
应用研究：探索基于TIM-3的免疫疗法，如开发特异性抗体或小分子抑制剂，以调控免疫反应。

总结

Human TIM-3在免疫调节中具有重要作用，其独特的结构和功能使其成为免疫学和疾病研究中的热点。未来，TIM-3可能在疾病诊断和治疗中发挥重要价值。

生物活性

Immobilized Human Galectin9 at 2 μg/mL can bind recombinant human HAVCR2 with a linear range of 0.3-5 μg/mL.

种属

Human

表达宿主

HEK293 cells

Calculated MW

46.66 kDa

Observed MW

65-75 kDa

标签

C-hFc&His

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Recombinant Human TIM-3/HAVCR2/CD366 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser22-Arg200 ) of human TIM-3 (Accession #NP_116171.3) fused with an Fc, 6×His tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin9 at 2 μg/mL can bind recombinant human HAVCR2 with a linear range of 0.3-5 μg/mL.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

背景信息

HAVCR2 also known as TIM3(T cell immunoglobulin and mucin domain-3), is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. An alternatively spliced isoform is truncated within the mucin-like stalk.TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity . TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA. TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity.

基因ID

84868

Swiss Prot

Q8TDQ0

别名

HAVCR2;CD366;HAVcr-2;KIM-3;TIM3;TIMD-3;TIMD3;Tim-3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2