种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human DNAM-1/CD226 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu19-Asn247) of human CD226 (Accession #NP_006557.2) fused with an Fc, 6×His tag at the C-terminus.
标签
C-hFc&His
纯度
> 90% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD226 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD155 with a linear range of 0.3-1.2 μg/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
研究方向
DNAM-1 蛋白与细胞毒性 T 淋巴细胞 (CTL) 和自然杀伤 (NK) 细胞介导的细胞间粘附、淋巴细胞信号传导、细胞毒性和淋巴因子分泌密切相关。DNAM-1 作为 NECTIN2 的细胞表面受体，在与配体结合后，刺激 T 细胞增殖和细胞因子（如 IL2、IL5、IL10、IL13 和 IFNG）的产生。此外，DNAM-1 与 PVRIG 竞争 NECTIN2 结合，强调了其在细胞相互作用和信号通路中的调节作用。该蛋白还与 PVR 和 NECTIN2 相互作用，有助于其在免疫反应和细胞通讯中的多方面功能。
背景信息
This protein is also known as PTA1 or DNAM-1, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. High rate of CD226 (Cluster of Differentiation 226) is found on the surface of natural killer cells, platelets, monocytes and a subset of T cells. CD226 have binding sites with CD112 and CD155 and mediate cellular adhesion to other cells containing its ligands.