活性重组蛋白 Recombinant Human CD10/MME Protein　重组蛋白

种属

Human

表达宿主

HEK293 Cells

描述

Recombinant Human CD10/MME Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Tyr52-Trp750) of human CD10/MME (Accession #NP_000893.2) fused with a 6×His tag at the C-terminus.

标签

C-His

纯度

> 95% by SDS-PAGE.

内毒素

<1EU/μg

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH, (R&D Systems, Catalog # ES005). The specific activity is >1,500 pmoles/min/μg.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM MES, 100mM NaCl, 1mM ZnCl2, 10%glycerol, pH 6.5.

储存

Store the lyophilized protein at -20°C to -80°C for 12 months.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

研究方向

MME 蛋白表现出类似嗜热菌蛋白酶的特异性，主要作用于最多 30 个氨基酸的多肽。在生物学上至关重要，它在阿片肽（包括甲硫氨酸和亮氨酸脑啡肽）的降解中发挥着关键作用，这是通过 Gly-Phe 键的裂解实现的。此外，MME 催化缓激肽、P 物质和神经降压素肽的裂解，并且能够裂解血管紧张素-1、血管紧张素-2 和血管紧张素 1-9。该蛋白参与心房钠尿因子 (ANF) 和脑钠尿因子 (BNP(1-32)) 的降解。此外，MME 对皮肤预弹性纤维和弹性纤维表现出紫外线诱导的弹性蛋白酶活性。

背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

基因ID

4311

Swiss Prot

P08473

别名

MME; EPN