种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human BACE-1/ASP2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Thr22-Tyr460) of human BACE1/ASP2 (Accession #NP_036236.1) fused with an 8×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >5.5 pmol/min/μg.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
研究方向
BACE1 是天冬氨酸蛋白酶肽酶 A1 家族的成员，在阿尔茨海默病病理学中发挥着关键作用。通过选择性剪接，该基因产生多种转录变体，其中至少一种编码前蛋白原，该前蛋白原经过蛋白水解加工产生成熟的蛋白酶。作为一种跨膜蛋白酶，BACE1 催化从淀粉样前体蛋白产生淀粉样β肽的初始步骤。β 淀粉样肽是阿尔茨海默病患者大脑中积累的β 淀粉样斑块的主要成分。BACE1 的表达很广泛，在多种组织中普遍存在，包括胰腺、大脑和 24 种其他组织，强调了其在不同生理环境中的重要性。
背景信息
Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells.BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein beta site in the brain to generate the amyloid beta peptide. Because the amyloid beta peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues,such brain, pancreatic tissue.