种属
Human
表达宿主
E. coli
描述
Recombinant Human MANF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Leu25-Leu182) of human MANF (Accession #NP_006001.4).
标签
No tag
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
研究方向
ARMET/MANF 蛋白选择性促进中脑腹侧多巴胺能神经元的存活，从而在神经元稳态中发挥至关重要的作用。它调节 GABA 能向黑质中多巴胺能神经元的传递，增强自发和诱发的 GABA 能抑制性突触后电流，表明其参与神经传递调节（通过相似性）。此外，ARMET/MANF 抑制细胞增殖并防止内质网 (ER) 应激诱导的细胞死亡，证明其在细胞过程中的多方面作用。在正常情况下，该蛋白通过与内质网伴侣蛋白 HSPA5 结合而保留在 ER/肌浆网 (SR) 中。然而，为了应对 ER 应激和缺氧，ARMET/MANF 被 ER/SR 上调和分泌，导致其与 3-O-磺基半乳糖神经酰胺（靶细胞外细胞膜中的一种脂硫脂）结合。这种相互作用通过内吞作用促进细胞摄取，对于 ARMET/MANF 在缺氧和 ER 应激条件下减轻 ER 应激和减轻细胞毒性的作用至关重要。该蛋白还直接与 HSPA5 相互作用，并且是涉及 CRELD2、MANF、MATN3 和 PDIA4 的复合物的一部分，进一步强调了其参与细胞调节网络（通过相似性）。
背景信息
The protein is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this protein increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in Normal tissues and are thus no longer thought to be tumor-related.