种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human Alkaline phosphatase Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.；It contains Val20-Asp503.
标签
N-His&Flag
纯度
> 95% as determined by Tris-Bis PAGE；> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
研究方向
碱性磷酸酶 (ALPL) 是一种前蛋白原，经过蛋白水解裂解产生信号肽和原蛋白，随后经过加工生成活性成熟肽。ALPL 也是一种膜结合糖基化酶，可在碱性 pH 条件下催化磷酸酯的水解。成熟肽维持骨矿化所需的无机磷酸盐与无机焦磷酸盐的比例。缺乏 ALPL 的小鼠会出现骨软化、骨骼软化的症状。在人类中，该基因的突变与低磷酸酯酶症有关，低磷酸酯酶症是一种遗传性代谢性骨病，缺乏这种酶会抑制骨矿化，导致骨骼缺陷。ALPL 在矿化组织细胞中高度表达，在硬组织的形成中发挥关键作用。ALPL 会增加无机磷酸盐的局部比率，促进矿化，并降低细胞外焦磷酸盐浓度 (矿物质形成的抑制剂)。此外，ALPL 主要通过调节无机磷酸盐 (Pi) 和无机焦磷酸盐 (PPi) 之间的平衡来刺激矿化，并在心血管重塑中发挥作用。
背景信息
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.