种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asn26-Ala423) of human Serpin A3/AACT (Accession #NP_001076.2) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <11.5 nM.
制剂
Lyophilized from a 0.22 μm filtered solution of 20mM HEPES,150mM NaCl, pH 8.0.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
研究方向
Serpin A3 蛋白是丝氨酸蛋白酶抑制剂家族的成员，具有丝氨酸蛋白酶抑制剂的作用。该基因位于 14 号染色体的 q 臂上，是丝氨酸蛋白酶抑制剂基因簇的一部分。Serpin A3 的多态性表现出组织特异性模式并影响蛋白酶靶向。该蛋白质序列的变异与阿尔茨海默病有关，其缺乏与肝脏疾病有关。值得注意的是，在帕金森病和慢性阻塞性肺病患者中已发现 Serpin A3 突变，强调了其在多种生理条件下的潜在作用。偏向的表达谱在肝脏 (RPKM 989.5)、胆囊 (RPKM 89.2) 和其他四种组织中显着存在，强调了其在各种生物学背景中的重要性。
背景信息
SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors. It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Serpin A3 is a major constituent of the plaques associated with Alzheimer’s disease and an inhibitor of amyloid beta peptide degradation.