种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human WAIF1/5T4/TPBG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser32-Ser355) of human TPBG/5T4 (Accession #NP_006661.1) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
研究背景
TPBG/5T4 蛋白似乎可作为 Wnt/β-连环蛋白信号传导的抑制剂，可能通过与 LRP6 间接相互作用来实现，从而阻止 Wnt3a 依赖性 LRP6 内化。这意味着 TPBG/5T4 在调节复杂的 Wnt/β-catenin 信号通路中发挥着至关重要的作用，并对与该通路相关的细胞反应产生调节影响。进一步探索控制 TPBG/5T4 和 LRP6 之间相互作用的特定分子机制，及其对 Wnt3a 依赖性 LRP6 内化的影响，可以对 TPBG/5T4 在塑造 Wnt/β-catenin 信号动力学中的功能意义产生有价值的见解及其对各种细胞过程的潜在影响。