| Catalog Number: | GX00647 |
| Swiss Prot： | Q9Y336 |
| Species: | Human |
| Expression System: | 293F |
| Sequence: | Gln18-Gly348 |
| Tag: | C-hFc&His tag |
| Purity: | >95% |
| Endotoxin: | ＜0.1EU/ug |
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| Product Name: | Active Recombinant Human SIGLEC9 Protein |
| Synonyms: | CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; Siglec9; Siglec-9 |
| Catalog Number: | GX00647 |
| Swiss Prot: | Q9Y336 |
| Species: | Human |
| Expression System: | 293F |
| Sequence: | Gln18-Gly348 |
| Tag: | C-hFc&His tag |
| Purity: | >95% |
| SDS Page: | 90-100 kDa |
| Endotoxin: | ＜0.1EU/ug |
| Carrier Free: | Yes |
| Animal Free: | Yes |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Description: | Recombinant Human SIGLEC9 Protein is produced by 293F expression system. The target Protein is expressed with Sequence (Gln18-Gly348) of (Accession Q9Y336) fused with a C-hFc&His tag |
| Activity: | Measured by the ability of the immobilized protein to support the adhesion of human red blood cells.The ED50 for this effect is 10.0-100 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| Storage: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
研究方向
Siglec-9 蛋白被鉴定为假定的粘附分子，充当唾液酸依赖性细胞结合的介质。它表现出优先结合 α-2,3- 或 α-2,6- 连接的唾液酸。值得注意的是，Siglec-9 的唾液酸识别位点可能由于与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。
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