| Catalog Number: | GX00023 |
| Swiss Prot： | O08665 |
| Species: | Mouse |
| Expression System: | 293F |
| Sequence: | Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg |
| Tag: | C-hFc&His tag |
| Purity: | ＞90% |
| Endotoxin: | ＜0.1EU/ug |
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| Product Name: | Active Recombinant Mouse Semaphorin 3A Protein |
| Synonyms: | (semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphor |
| Catalog Number: | GX00023 |
| Swiss Prot: | O08665 |
| Species: | Mouse |
| Expression System: | 293F |
| Sequence: | Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg |
| Tag: | C-hFc&His tag |
| Purity: | ＞90% |
| SDS Page: | 115- 120 kDa |
| Endotoxin: | ＜0.1EU/ug |
| Carrier Free: | Yes |
| Animal Free: | Yes |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Description: | Recombinant Mouse Semaphorin 3A Protein is produced by 293F expression system. The target Protein is expressed with Sequence (Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala) of Mouse Semaphorin 3A(Accession O08665) fused with a C-hFc&His tag |
| Activity: | Measured by its ability to cause collapse of chick embryonic dorsalroot ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Mouse Semaphorin 3A Fc Chimera causes ＞50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta ‑NGF. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| Storage: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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