| Catalog Number: | GX00505 |
| Swiss Prot: | Q8IU57 |
| Species: | Human |
| Expression System: | 293F |
| Sequence: | 21- 228 aa |
| Tag: | C-hFc&His tag |
| Purity: | >95% |
| Endotoxin: | <0.1EU/ug |
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500
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| Product Name: | Active Recombinant Human IL28RA Protein |
| Synonyms: | class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; |
| Catalog Number: | GX00505 |
| Swiss Prot: | Q8IU57 |
| Species: | Human |
| Expression System: | 293F |
| Sequence: | 21- 228 aa |
| Tag: | C-hFc&His tag |
| Purity: | >95% |
| SDS Page: | 65- 75 kDa |
| Endotoxin: | <0.1EU/ug |
| Carrier Free: | Yes |
| Animal Free: | Yes |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Description: | Recombinant Human IL28RA Protein is produced by 293F expression system. The target Protein is expressed with Sequence (21- 228 aa) of (Accession Q8IU57) fused with a C-hFc&His tag |
| Activity: | Measured by its binding ability in a functional ELISA. In a 100 μL reaction mixture containing rhIL-28 R alpha /Fc Chimera at 1 μg/mL and rhIL- 28A dilutions at 1- 1000 ng/mL, the concentration of rhIL-28A that produces 50% of the optimal binding response is found to be approximately 40- 200 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。 |
| Storage: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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