Recombinant Rat PPM1K Protein
| 货号： | GXP84313 |
|---|---|
| Swiss Prot： | A6K136 |
| 种属： | Rat |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 30–372aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
价格：¥2580
货期：3-4周/weeks
规格：
说明书：
产品详情
| 产品名称： | Recombinant Rat PPM1K Protein |
|---|---|
| 别名： | Ppm1k |
| 货号： | GXP84313 |
| Swiss Prot： | A6K136 |
| 种属： | Rat |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 30–372aa |
| 标签： | Customize |
| 纯度： | >90% |
| 实际分子量： | Depends On Tags |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Serine/threonine-protein phosphatase component of macronutrients metabolism. Together with BCKDK serves as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) and protein synthesis with glucose and lipid metabolism via two distinct phosphoprotein targets: BCKDHA/E1a subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and ACLY, a lipogenic enzyme of Krebs cycle (By similarity) (PubMed:29779826). At high levels of branched-chain ketoacids (BCKAs), dephosphorylates and activates mitochondrial BCKDH complex, a multisubunit complex consisting of three components, heterotetrameric E1 composed of BCKDHA and BCKDHB chains, 24-meric E2 core composed of DBT and homodimeric E3 composed of DLD, each involved in different steps of BCAA catabolism. Tightly associates with the E2 subunit of BCKDH complex and dephosphorylates Ser-333 of BCKDHA chain of the E1 subunit likely through on-off binding to individual E2 subunits of the 24-meric E2 core to increase the efficiency of the dephosphorylation reaction (By similarity). Appears to dephosphorylate and inactivate cytosolic ACLY in response to changes of cellular carbohydrate abundance. Overnutrition and in particular high-fructose diet, activates MLXIPL/ChREBP leading to increased BCKDK to PPM1K ratio, phosphorylation of ACLY on Ser-454 and activation of its enzymatic activity that ultimately results in the generation of acetyl-CoA and malonyl-CoA immediate substrates of de novo lipogenesis (By similarity) (PubMed:29779826). Recognizes phosphosites having SxS or RxxS motifs and strictly depends on Mn(2+) ions for the phosphatase activity (By similarity). Regulates Ca(2+)-induced opening of mitochondrial transition pore and apoptotic cell death (By similarity). {ECO:0000250|UniProtKB:Q8N3J5, ECO:0000269|PubMed:29779826}. |
| 活性测试： | Not Test |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
