
| 货号: | GXP56957 |
|---|---|
| Swiss Prot: | Q9CR84 |
| 种属: | Mouse |
| 表达宿主: | E. Coli / 293F |
| 表达区间: | 62–136aa |
| 标签: | Customize |
| 纯度: | >90% |
| 内毒素: | < 1.0 EU/μg / < 0.1 EU/μg |
| 产品名称: | Recombinant Mouse AT5G1 Protein |
|---|---|
| 别名: | Atp5mc1 Atp5g1 |
| 货号: | GXP56957 |
| Swiss Prot: | Q9CR84 |
| 种属: | Mouse |
| 表达宿主: | E. Coli / 293F |
| 表达区间: | 62–136aa |
| 标签: | Customize |
| 纯度: | >90% |
| 实际分子量: | Depends On Tags |
| 内毒素: | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体: | Yes |
| 无动物源: | Yes |
| 制剂: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述: | Subunit c, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain – the catalytic core – and a membrane F(1) domain – the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. With the subunit a (MT-ATP6), forms the proton-conducting channel in the F(0) domain, that contains two crucial half-channels (inlet and outlet) that facilitate proton movement from the mitochondrial intermembrane space (IMS) into the matrix. Protons are taken up via the inlet half-channel and released through the outlet half-channel, following a Grotthuss mechanism. {ECO:0000250|UniProtKB:P05496}. |
| 活性测试: | Not Test |
| 复溶: |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。 |
| 储存: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。 |
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