Recombinant E. coli DnaK / Hsp70 Protein

货号: GX01904
Swiss Prot: P0A6Y8
种属: E.coli
表达宿主: E.coli
表达区间: 385-546aa
标签: N-6His
纯度: >90%
内毒素: <1.0EU/ug
价格:¥500
货期:2-3 周/Weeks
规格:

说明书:

产品详情

产品名称: Recombinant E. coli DnaK / Hsp70 Protein
别名: Chaperone protein dnaK, HSP70, groP, grpF, seg
货号: GX01904
Swiss Prot: P0A6Y8
种属: E.coli
表达宿主: E.coli
表达区间: 385-546aa
标签: N-6His
纯度: >90%
实际分子量: 17.7kDa (163aa)
内毒素: <1.0EU/ug
无载体: Yes
无动物源: Yes
制剂: 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM EDTA,2 mM -mercaptoethanol
描述: Recombinant E.coli DnakProtein is produced by E.coli expression system. The target Protein is expressed with Sequence (385-546aa) of (Accession P0A6Y8) fused with a N-6His
活性测试: 待测
复溶: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
储存: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
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