Active Recombinant Human BTN3A1 / CD277 Protein
| 货号： | GX00020 |
|---|---|
| Swiss Prot： | O00481 |
| 种属： | Human |
| 表达宿主： | 293F |
| 表达区间： | Gln30-Gly254 |
| 标签： | C-hFc&His tag |
| 纯度： | ＞95% |
| 内毒素： | ＜0.1EU/ug |
价格：¥500
货期：现货/In Stock
规格：
说明书：
产品详情
| 产品名称： | Active Recombinant Human BTN3A1 / CD277 Protein |
|---|---|
| 别名： | BT3.1BTF5BTN3A1butyrophilin, subfamily 3, member A1CD277dJ45P21.3 (butyrophilin, subfamily 3, member A1) |
| 货号： | GX00020 |
| Swiss Prot： | O00481 |
| 种属： | Human |
| 表达宿主： | 293F |
| 表达区间： | Gln30-Gly254 |
| 标签： | C-hFc&His tag |
| 纯度： | ＞95% |
| 实际分子量： | 60kDa |
| 内毒素： | ＜0.1EU/ug |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Recombinant Human BTN3A1 Protein is produced by 293F expression system. The target Protein is expressed with Sequence (Gln30-Gly254) of Human BTN3A1(Accession O00481) fused with a C-terminal Fc, 6×His tag |
| 活性测试： | Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat. The ED50 for this effect is 20-100 ng/mL. |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
