Recombinant Mouse PK3CG Protein
| 货号： | GXP57604 |
|---|---|
| Swiss Prot： | Q9JHG7 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 1–1102aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
价格：¥2580
货期：3-4周/weeks
规格：
说明书：
产品详情
| 产品名称： | Recombinant Mouse PK3CG Protein |
|---|---|
| 别名： | Pik3cg Pi3kg1 |
| 货号： | GXP57604 |
| Swiss Prot： | Q9JHG7 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 1–1102aa |
| 标签： | Customize |
| 纯度： | >90% |
| 实际分子量： | Depends On Tags |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:10669418, PubMed:11054537). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology (PubMed:10669416). Links G-protein coupled receptor activation to PIP3 production (PubMed:10669416). Involved in immune, inflammatory and allergic responses (PubMed:10669416, PubMed:10669418, PubMed:16127437, PubMed:31554793). Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge (PubMed:10669416). Controls motility of dendritic cells (PubMed:15318168). Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation (PubMed:16116162, PubMed:19297623). Participates in T-lymphocyte migration (PubMed:19297623). Regulates T-lymphocyte proliferation, activation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis (PubMed:17673465). Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism (PubMed:17673465). May have also a lipid kinase activity-dependent function in platelet aggregation (PubMed:17673465). Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility (PubMed:12297047). Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels (PubMed:12297047, PubMed:15294162, PubMed:21474070). Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation (PubMed:21474070). Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis (By similarity). May also have a scaffolding role in modulating cardiac contractility. Contribute to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity). In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway downstream of RASGRP4-mediated Ras-activation, to promote neutrophil functional responses (PubMed:22728827). {ECO:0000250|UniProtKB:P48736, ECO:0000269|PubMed:10669416, ECO:0000269|PubMed:10669418, ECO:0000269|PubMed:11054537, ECO:0000269|PubMed:12297047, ECO:0000269|PubMed:15294162, ECO:0000269|PubMed:15318168, ECO:0000269|PubMed:16116162, ECO:0000269|PubMed:16127437, ECO:0000269|PubMed:17673465, ECO:0000269|PubMed:19297623, ECO:0000269|PubMed:21474070, ECO:0000269|PubMed:22728827, ECO:0000269|PubMed:31554793}. |
| 活性测试： | ACTIVITY REGULATION: Activated by both the alpha and the beta-gamma G proteins following stimulation of G protein-coupled receptors (GPCRs). Activation by GPCRs is assisted by the regulatory subunit PIK3R5 leading to the translocation from the cytosol to the plasma membrane and to kinase activation; the respective activation involving PIK3R6 requires HRAS for membrane recruitment. Wortmannin sensitive in nM range. Inhibited by AS252424. {ECO:0000269|PubMed:11054537, ECO:0000269|PubMed:19297623}. |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
