
| 货号: | GXP57157 |
|---|---|
| Swiss Prot: | Q9D379 |
| 种属: | Mouse |
| 表达宿主: | E. Coli / 293F |
| 表达区间: | 1–455aa |
| 标签: | Customize |
| 纯度: | >90% |
| 内毒素: | < 1.0 EU/μg / < 0.1 EU/μg |
| 产品名称: | Recombinant Mouse HYEP Protein |
|---|---|
| 别名: | Ephx1 |
| 货号: | GXP57157 |
| Swiss Prot: | Q9D379 |
| 种属: | Mouse |
| 表达宿主: | E. Coli / 293F |
| 表达区间: | 1–455aa |
| 标签: | Customize |
| 纯度: | >90% |
| 实际分子量: | Depends On Tags |
| 内毒素: | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体: | Yes |
| 无动物源: | Yes |
| 制剂: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述: | Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol (By similarity). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (PubMed:34799735). {ECO:0000250|UniProtKB:P07099, ECO:0000250|UniProtKB:P07687, ECO:0000269|PubMed:34799735}. |
| 活性测试: | ACTIVITY REGULATION: Inhibited by 10-hydroxystearamide and methoxy-arachidonyl fluorophosphate. {ECO:0000250|UniProtKB:P07099}. |
| 复溶: |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。 |
| 储存: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。 |
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