Recombinant Mouse GPR68 Protein
| 货号： | GXP54911 |
|---|---|
| Swiss Prot： | Q8BFQ3 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 1–365aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
价格：¥2580
货期：3-4周/weeks
规格：
说明书：
产品详情
| 产品名称： | Recombinant Mouse GPR68 Protein |
|---|---|
| 别名： | Gpr68 Ogr1 |
| 货号： | GXP54911 |
| Swiss Prot： | Q8BFQ3 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 1–365aa |
| 标签： | Customize |
| 纯度： | >90% |
| 实际分子量： | Depends On Tags |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions (By similarity). The receptor is almost silent at pH 7.8 but fully activated at pH 6.8 (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as phospholipase C (By similarity). GPR68 is mainly coupled to G(q) G proteins and mediates production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (By similarity). Acts as a key mechanosensor of fluid shear stress and membrane stretch (PubMed:29677517). Expressed in endothelial cells of small-diameter resistance arteries, where it mediates flow-induced dilation in response to shear stress (PubMed:29677517). May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone (PubMed:18847331). Acts as a regulator of calcium-sensing receptor CASR in a seesaw manner: GPR68-mediated signaling inhibits CASR signaling in response to protons, while CASR inhibits GPR68 in presence of extracellular calcium (PubMed:26261299). Also functions as a metastasis suppressor gene in prostate cancer (PubMed:17728215). {ECO:0000250|UniProtKB:Q15743, ECO:0000269|PubMed:17728215, ECO:0000269|PubMed:18847331, ECO:0000269|PubMed:26261299, ECO:0000269|PubMed:29677517}. |
| 活性测试： | ACTIVITY REGULATION: Activated by a network of residues that connects an extracellular-facing cavity to Glu-149, a conserved charged residue buried in the transmembrane core of the receptor (By similarity). Protonation likely drives conformational changes in extracellular loop 2 (ECL2), which stabilizes movement of transmembrane 3 (TM3) and a series of rearrangements that connect the extracellular-facing cavity to Glu-149, a residue only conserved in proton-sensing G-protein coupled receptors (By similarity). Activated in an allosteric manner by divalent metal ions at the extracellular surface following the order: Cd(2+) > Co(2+) > Ni(2+) > Zn(2+) > Fe(2+) > Ca(2+) > Mg(2+) (By similarity). Activated by ogerin (ZINC67740571), a selective GPR68 positive allosteric modulator (PubMed:26550826). Inhibited by small molecule GPR68-I, decreasing inflammation in models of colitis (PubMed:34722644). {ECO:0000250|UniProtKB:Q15743, ECO:0000269|PubMed:26550826, ECO:0000269|PubMed:34722644}. |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
