Recombinant Mouse GFRAL Protein
| 货号： | GXP54433 |
|---|---|
| Swiss Prot： | Q6SJE0 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 20–393aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
价格：¥2580
货期：3-4周/weeks
规格：
说明书：
产品详情
| 产品名称： | Recombinant Mouse GFRAL Protein |
|---|---|
| 别名： | Gfral Gral |
| 货号： | GXP54433 |
| Swiss Prot： | Q6SJE0 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 20–393aa |
| 标签： | Customize |
| 纯度： | >90% |
| 实际分子量： | Depends On Tags |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:31152154, PubMed:31928886, PubMed:32723474, PubMed:33593916, PubMed:35202387, PubMed:36630958, PubMed:37380764). The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:31928886, PubMed:32723474, PubMed:33593916, PubMed:35202387, PubMed:36630958). The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin (PubMed:31875646, PubMed:31928886, PubMed:32661391, PubMed:32723474). Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways (PubMed:28846098, PubMed:28846099, PubMed:32723474, PubMed:33593916). Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed:28846098, PubMed:28846099, PubMed:31152154, PubMed:32723474). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PubMed:32661391). {ECO:0000269|PubMed:28846097, ECO:0000269|PubMed:28846098, ECO:0000269|PubMed:28846099, ECO:0000269|PubMed:28953886, ECO:0000269|PubMed:31152154, ECO:0000269|PubMed:31875646, ECO:0000269|PubMed:31928886, ECO:0000269|PubMed:32661391, ECO:0000269|PubMed:32723474, ECO:0000269|PubMed:33593916, ECO:0000269|PubMed:35202387, ECO:0000269|PubMed:36630958, ECO:0000269|PubMed:37380764}. |
| 活性测试： | ACTIVITY REGULATION: Specifically inhibited by 3P10 monoclonal antibody (PubMed:32661391). Strongly activated by LY3463251, a long-acting and stable agonist composed of GDF15 conjugated monomeric human IgG4 Fc (PubMed:36630958). {ECO:0000269|PubMed:32661391, ECO:0000269|PubMed:36630958}. |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
