Recombinant Mouse PIEZ1 Protein
| 货号： | GXP50273 |
|---|---|
| Swiss Prot： | E2JF22 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 1–2547aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
价格：¥2580
货期：3-4周/weeks
规格：
说明书：
产品详情
| 产品名称： | Recombinant Mouse PIEZ1 Protein |
|---|---|
| 别名： | Piezo1 Fam38a |
| 货号： | GXP50273 |
| Swiss Prot： | E2JF22 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 1–2547aa |
| 标签： | Customize |
| 纯度： | >90% |
| 实际分子量： | Depends On Tags |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain (PubMed:20813920, PubMed:22343900, PubMed:38184690, PubMed:26390154, PubMed:29261642, PubMed:35388220, PubMed:37590348). Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways (PubMed:26390154, PubMed:29261642, PubMed:35388220, PubMed:37590348). Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium (PubMed:20813920, PubMed:22343900, PubMed:38228630). Conductance to monovalent alkali ions is highest for K(+), intermediate for Na(+) and lowest for Li(+) (By similarity). Divalent ions except for Mn(2+) permeate the channel but more slowly than the monovalent ions and they also reduce K(+) currents (By similarity). Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling (By similarity). In inner ear hair cells, PIEZO1/2 subunits may constitute part of the mechanotransducer (MET) non-selective cation channel complex where they may act as pore-forming ion-conducting component in the complex (PubMed:38228630). In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing (PubMed:24157948). Acts as a shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation (By similarity). Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (PubMed:24958852, PubMed:25119035, PubMed:36515266). Acts as a sensor of phosphatidylserine (PS) flipping at the plasma membrane and governs morphogenesis of muscle cells (PubMed:29799007). In myoblasts, flippase-mediated PS enrichment at the inner leaflet of plasma membrane triggers channel activation and Ca(2+) influx followed by Rho GTPases signal transduction, leading to assembly of cortical actomyosin fibers and myotube formation (PubMed:29799007). {ECO:0000250|UniProtKB:Q92508, ECO:0000269|PubMed:20813920, ECO:0000269|PubMed:22343900, ECO:0000269|PubMed:24157948, ECO:0000269|PubMed:24958852, ECO:0000269|PubMed:25119035, ECO:0000269|PubMed:26390154, ECO:0000269|PubMed:29261642, ECO:0000269|PubMed:29799007, ECO:0000269|PubMed:35388220, ECO:0000269|PubMed:36515266, ECO:0000269|PubMed:37590348, ECO:0000269|PubMed:38184690, ECO:0000269|PubMed:38228630}. |
| 活性测试： | ACTIVITY REGULATION: Regulated by auxillary subunits MDFIC and MDFI (PubMed:37590348). Down-regulated by phosphatidylserines exposed on the cell surface (PubMed:29799007). Divalent ions decrease the single-channel permeability of K(+) (By similarity). {ECO:0000250|UniProtKB:Q92508, ECO:0000269|PubMed:29799007, ECO:0000269|PubMed:37590348}. |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
