Recombinant Mouse PNDC1 Protein

Catalog Number: GXP50207
Swiss Prot: B2RXZ1
Species: Mouse
Expression System: E. Coli / 293F
Sequence: 1–531aa
Tag: Customize
Purity: >90%
Endotoxin: < 1.0 EU/μg / < 0.1 EU/μg
Price:¥2580
Delivery Time:3-4周/weeks
Specifications:

Manual:

Product Details

Product Name: Recombinant Mouse PNDC1 Protein
Synonyms: Pnldc1
Catalog Number: GXP50207
Swiss Prot: B2RXZ1
Species: Mouse
Expression System: E. Coli / 293F
Sequence: 1–531aa
Tag: Customize
Purity: >90%
SDS Page: Depends On Tags
Endotoxin: < 1.0 EU/μg / < 0.1 EU/μg
Carrier Free: Yes
Animal Free: Yes
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails (PubMed:27515512). Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development (PubMed:27515512). May act as a regulator of multipotency in embryonic stem cells (PubMed:27515512). Is a critical factor for proper spermatogenesis, involved in pre-piRNAs processing to generate mature piRNAs (By similarity). {ECO:0000250|UniProtKB:Q8NA58, ECO:0000269|PubMed:27515512}.
Activity: Not Test
Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
Storage: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
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