Recombinant Mouse UBP36 Protein
| Catalog Number： | GXP50167 |
|---|---|
| Swiss Prot： | B1AQJ2 |
| Species： | Mouse |
| Expression System： | E. Coli / 293F |
| Sequence： | 1–1098aa |
| Tag： | Customize |
| Purity： | >90% |
| Endotoxin： | < 1.0 EU/μg / < 0.1 EU/μg |
Price：¥2580
Delivery Time：3-4周/weeks
Specifications：
Manual：
Product Details
| Product Name： | Recombinant Mouse UBP36 Protein |
|---|---|
| Synonyms： | Usp36 Kiaa1453 |
| Catalog Number： | GXP50167 |
| Swiss Prot： | B1AQJ2 |
| Species： | Mouse |
| Expression System： | E. Coli / 293F |
| Sequence： | 1–1098aa |
| Tag： | Customize |
| Purity： | >90% |
| SDS Page： | Depends On Tags |
| Endotoxin： | < 1.0 EU/μg / < 0.1 EU/μg |
| Carrier Free： | Yes |
| Animal Free： | Yes |
| Formulation： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability (PubMed:29273634). Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634). Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (By similarity). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex (By similarity). In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm (By similarity). Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions (By similarity). Deubiquitinates SOD2, regulates SOD2 protein stability (By similarity). Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (By similarity). Promotes CEP63 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability (By similarity). Acts as a SUMO ligase to promote EXOSC10 sumoylation critical for the nucleolar RNA exosome function in rRNA processing (By similarity). Binds to pre-rRNAs (By similarity). {ECO:0000250|UniProtKB:Q9P275, ECO:0000269|PubMed:29273634}. |
| Activity： | Not Test |
| Reconstitution： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| Storage： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
