Active Recombinant Human Chitinase 3-like 2 / CHI3L2 Protein
| Catalog Number： | GX00433 |
|---|---|
| Swiss Prot： | Q15782 |
| Species： | Human |
| Expression System： | 293F |
| Sequence： |
27- 390 aa |
| Tag： |
C- 10*His tag |
| Purity： | >95% |
| Endotoxin： | ＜0.1EU/ug |
Price：¥500
Delivery Time：现货/In Stock
Specifications：
Manual：
Product Details
| Product Name： | Active Recombinant Human Chitinase 3-like 2 / CHI3L2 Protein |
|---|---|
| Synonyms： | CH3L2_HUMANCHI3L2Chitinase 3 like 2Chitinase-3-like protein 2Chondrocyte protein 39OTTHUMP00000014028YKL 39YKL-39YKL39 |
| Catalog Number： | GX00433 |
| Swiss Prot： | Q15782 |
| Species： | Human |
| Expression System： | 293F |
| Sequence： |
27- 390 aa |
| Tag： |
C- 10*His tag |
| Purity： | >95% |
| SDS Page： |
40 kDa |
| Endotoxin： | ＜0.1EU/ug |
| Carrier Free： | Yes |
| Animal Free： | Yes |
| Formulation： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： |
Recombinant Human Chitinase 3-like 2 Protein is produced by 293F expression system. The target Protein is expressed with Sequence (27- 390 aa) of (Accession Q15782) fused with a C- 10*His tag |
| Activity： | Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cellsWhen 5 x 10^4 cells per well are added to rhCHI3L2 coated plates for 30 minutes at 37 °C, cell adhesion is enhanced in a dose dependent manner with typical ED50 range of 0.7-2.8 μg/mL. Opitimal dilutions should be determined by each laboratory for each application. |
| Reconstitution： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| Storage： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
