Active Recombinant Human MMP-15 / MT2-MMP Protein

Catalog Number: GX00336
Swiss Prot: P51511
Species: Human
Expression System: 293F
Sequence: Glu47-
Pro565(Arg128Pro,Arg129Gly)
Tag: C-5*His tag
Purity: >90%
Endotoxin: <0.1EU/ug
Price:¥500
Delivery Time:现货/In Stock
Specifications:

Manual:

Product Details

Product Name: Active Recombinant Human MMP-15 / MT2-MMP Protein
Synonyms: matrix metallopeptidase 15 (membrane-inserted)matrix metalloproteinase 15 (membrane-inserted)Membrane-type matrix metalloproteinase 2Membrane-type-2 matrix metalloproteinaseMMP15MMP-15MT2-MMPMT2-MMPMT2MMPMTMMP2MT-MMP 2SMCP-2matrix metalloproteinase-15
Catalog Number: GX00336
Swiss Prot: P51511
Species: Human
Expression System: 293F
Sequence: Glu47-
Pro565(Arg128Pro,Arg129Gly)
Tag: C-5*His tag
Purity: >90%
SDS Page: 60-65 kDa
Endotoxin: <0.1EU/ug
Carrier Free: Yes
Animal Free: Yes
Formulation: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述: Recombinant Human
MMP-15/MT2-MMP Protein is produced by 293F expression system. The target Protein is expressed with Sequence ( Glu47-
Pro565(Arg128Pro,Arg129Gly)) of (Accession P51511) fused with a C-5*His tag
Activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is >200 pmol/min/μg, as measured underthe described conditions.
Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
Storage: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
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