Active Recombinant Mouse CD40 Ligand / CD154 / TNFSF5 Protein
| Catalog Number： | GX00262 |
|---|---|
| Swiss Prot： | P27548 |
| Species： | Mouse |
| Expression System： | 293F |
| Sequence： |
115- 260 aa |
| Tag： | C-His |
| Purity： | >95% |
| Endotoxin： | ＜0.1EU/ug |
Price：¥500
Delivery Time：现货/In Stock
Specifications：
Manual：
Product Details
| Product Name： | Active Recombinant Mouse CD40 Ligand / CD154 / TNFSF5 Protein |
|---|---|
| Synonyms： | CD40 ligandCD40-LTRAPCD154sCD40LTNFSF5 |
| Catalog Number： | GX00262 |
| Swiss Prot： | P27548 |
| Species： | Mouse |
| Expression System： | 293F |
| Sequence： |
115- 260 aa |
| Tag： | C-His |
| Purity： | >95% |
| SDS Page： |
22-25 kDa |
| Endotoxin： | ＜0.1EU/ug |
| Carrier Free： | Yes |
| Animal Free： | Yes |
| Formulation： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： |
Recombinant Mouse CD40 Ligand/TNFSF5 Protein is produced by 293F expression system. The target Protein is expressed with Sequence (115- 260 aa ) of (Accession P27548) fused with a C-His |
| Activity： | Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. The ED50 for this effect is 0.07- 0.35 ng/mL in the presence of a cross‑linking antibody, Mouse AntiHemagglutinin/HA Peptide Monoclonal Antibody. |
| Reconstitution： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| Storage： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
