Recombinant Mouse CASR Protein
| 货号： | GXP57953 |
|---|---|
| Swiss Prot： | Q9QY96 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 20–1079aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
价格：¥2580
货期：3-4周/weeks
规格：
说明书：
产品详情
| 产品名称： | Recombinant Mouse CASR Protein |
|---|---|
| 别名： | Casr Gprc2a |
| 货号： | GXP57953 |
| Swiss Prot： | Q9QY96 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 20–1079aa |
| 标签： | Customize |
| 纯度： | >90% |
| 实际分子量： | Depends On Tags |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体： | Yes |
| 无动物源： | Yes |
| 制剂： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed:23143333). Senses fluctuations in the circulating calcium concentration: activated by elevated circulating calcium, leading to decreased parathyroid hormone (PTH) secretion in parathyroid glands (PubMed:7493018). In kidneys, acts as a key regulator of renal tubular calcium resorption (PubMed:12671051). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G-proteins) and modulates the activity of downstream effectors (By similarity). CASR is coupled with different G(q)/G(11), G(i)/G(o)- or G(s)-classes of G-proteins depending on the context (PubMed:23143333). In the parathyroid and kidney, CASR signals through G(q)/G(11) and G(i)/G(o) G-proteins: G(q)/G(11) coupling activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers, while G(i)/G(o) coupling mediates inhibition of adenylate cyclase activity (PubMed:23143333). The G-protein-coupled receptor activity is activated by a co-agonist mechanism: aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (By similarity). Acts as an activator of the NLRP3 inflammasome via G(i)/G(o)-mediated signaling: down-regulation of cyclic AMP (cAMP) relieving NLRP3 inhibition by cAMP (PubMed:23143333). Acts as a regulator of proton-sensing receptor GPR68 in a seesaw manner: CASR-mediated signaling inhibits GPR68 signaling in response to extracellular calcium, while GPR68 inhibits CASR in presence of extracellular protons (PubMed:26261299). {ECO:0000250|UniProtKB:P41180, ECO:0000269|PubMed:12671051, ECO:0000269|PubMed:23143333, ECO:0000269|PubMed:26261299, ECO:0000269|PubMed:7493018}. |
| 活性测试： | ACTIVITY REGULATION: In resting state, adopts an open conformation, anion-binding promoting the inactive configuration. Upon aromatic amino acid-binding, the groove in the extracellular venus flytrap module is closed, thereby inducing the formation of a novel homodimer interface between subunits. Calcium ions stabilize the active state by enhancing homodimer interactions between membrane-proximal domains to fully activate the receptor. Upon activation, the homodimer adopts an asymmetric configuration of the 7-transmembrane region that primes one protomer for G-protein coupling. G-protein binding expands the transmembrane dimer interface; the restriction imposed by the receptor dimer, in combination with intracellular loop 2 (ICL2), enables G-protein activation by facilitating conformational transition of G-protein alpha. Coupling to different classes of G-proteins results in distinct CASR-G-protein interfaces. {ECO:0000250|UniProtKB:P41180}. |
| 复溶： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| 储存： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
