Recombinant Mouse AT2C2 Protein
| Catalog Number： | GXP50158 |
|---|---|
| Swiss Prot： | A7L9Z8 |
| Species： | Mouse |
| Expression System： | E. Coli / 293F |
| Sequence： | 1–944aa |
| Tag： | Customize |
| Purity： | >90% |
| Endotoxin： | < 1.0 EU/μg / < 0.1 EU/μg |
Price：¥2580
Delivery Time：3-4周/weeks
Specifications：
Manual：
Product Details
| Product Name： | Recombinant Mouse AT2C2 Protein |
|---|---|
| Synonyms： | Atp2c2 Kiaa0703 Spca2 |
| Catalog Number： | GXP50158 |
| Swiss Prot： | A7L9Z8 |
| Species： | Mouse |
| Expression System： | E. Coli / 293F |
| Sequence： | 1–944aa |
| Tag： | Customize |
| Purity： | >90% |
| SDS Page： | Depends On Tags |
| Endotoxin： | < 1.0 EU/μg / < 0.1 EU/μg |
| Carrier Free： | Yes |
| Animal Free： | Yes |
| Formulation： | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述： | ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (By similarity). Induces Ca(2+) influx independently of its ATP-driven pump function. At the basolateral membrane of mammary epithelial cells, interacts with Ca(2+) channel ORAI1 and mediates Ca(2+) entry independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May facilitate transepithelial transport of large quantities of Ca(2+) for milk secretion via activation of Ca(2+) influx channels at the plasma membrane and active Ca(2+) transport at the Golgi apparatus (By similarity) (PubMed:23840669). {ECO:0000250|UniProtKB:O75185, ECO:0000269|PubMed:23840669}. |
| Activity： | Not Test |
| Reconstitution： |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。 |
| Storage： |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
