| 货号： | GXP50024 |
| Swiss Prot： | A1L314 |
| 种属： | Mouse |
| 表达宿主： | E. Coli / 293F |
| 表达区间： | 20--713aa |
| 标签： | Customize |
| 纯度： | >90% |
| 内毒素： | < 1.0 EU/μg / < 0.1 EU/μg |
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500 元
3-4周/weeks
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| 产品名称: | Recombinant Mouse MPEG1 Protein |
| 别名: | Mpeg1 |
| 货号: | GXP50024 |
| Swiss Prot: | A1L314 |
| 种属: | Mouse |
| 表达宿主: | E. Coli / 293F |
| 表达区间: | 20--713aa |
| 标签: | Customize |
| 纯度: | >90% |
| 实际分子量: | Depends On Tags |
| 内毒素: | < 1.0 EU/μg / < 0.1 EU/μg |
| 无载体: | Yes |
| 无动物源: | Yes |
| 制剂: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| 描述: | Pore-forming protein involved in both innate and adaptive immunity (PubMed:26402460, PubMed:30249808, PubMed:32064340, PubMed:36028507, PubMed:36245269, PubMed:37347855). Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation (PubMed:35471730, PubMed:37347855). Also involved in innate immune response following bacterial infection; shows antibacterial activity against |
| 活性测试: | ACTIVITY REGULATION: Forms arc- and ring-shaped pre-pores on top of the membrane at neutral to slightly acidic pH conditions and converts to pores upon acidification (PubMed:32064340, PubMed:36028507, PubMed:36245269). Undergoes transition from the pre-pore to the pore in a processive clockwise hand-over-hand process (PubMed:32064340, PubMed:36028507, PubMed:36245269). In the pore state, 2 alpha-helical regions refold into transmembrane hairpins (TMH1 and TMH2) in each protomer that form in the |
| 复溶: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. 收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-81°C，随取随用，避免反复冻融。 |
| 储存: |
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. 未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。 |
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