Active Recombinant Mouse IL-1R1 / CD121a Protein

货号: GX00174
Swiss Prot: P13504-1
种属: Mouse
表达宿主: 293F
表达区间: Leu20 – Lys338
标签: C-His
纯度: >90%
内毒素: <0.1EU/ug
价格:¥500
货期:现货/In Stock
规格:

说明书:

产品详情

产品名称: Active Recombinant Mouse IL-1R1 / CD121a Protein
别名: rMuInterleukin-1 receptor type 1/IL-1R1, His Interleukin-1 receptor type 1IL-1R-1IL-1RT1IL-1 RICD121a
货号: GX00174
Swiss Prot: P13504-1
种属: Mouse
表达宿主: 293F
表达区间: Leu20 – Lys338
标签: C-His
纯度: >90%
实际分子量: 55-60
kDa
内毒素: <0.1EU/ug
无载体: Yes
无动物源: Yes
制剂: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述: Recombinant Mouse IL1 ReceptorI/IL-1R-1 Protein is produced by 293F expression system. The target Protein is expressed with Sequence (Leu20 – Lys338) of (Accession P13504-1) fused with a C-His
活性测试: Measured by its ability to inhibit IL-1 alpha – dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.005‑0.015 µg/mL of IL-1 sRI will inhibit 50% of the biological response due to 50 pg/mL of rmIL-1a
复溶: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。
储存: Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
未开盖的干粉蛋白在 -20°C至-80°C可保存12个月; 复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
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